Journal: Nature Communications
Article Title: A molecular staging model for accurately dating the endometrial biopsy
doi: 10.1038/s41467-023-41979-z
Figure Lengend Snippet: Figure 4a. PCA plot using 266 endometrial samples coloured to show the molecular staging model cycle stage. The PCA plot has a characteristic pattern with the samples in cyclic pattern corresponding to approximate cycle stage order. Figure 4b. PCA plot using Illumina microarray data from GSE141549 , with samples identified as menstrual, proliferative, or secretory by the authors. Note significant mixing of proliferative samples with menstrual and secretory ones. Figure 4c. The same PCA plot using data from GSE141549 with cycle stage assigned by the molecular staging model. Note minimal overlap between different molecular staging model cycle stages. Figure 4d. PCA plot using RNA-seq data from GSE65099 with samples identified as LH + 6 to LH + 10 as reported in the study. Figure 4e. The same PCA plot using data from GSE65099 with cycle stage assigned by the molecular staging model. Note reassignment of 2 outlying samples on the PCA plot as proliferative and not secretory. Source data are provided as a Source Data file.
Article Snippet: To further explore the effects of aging on endometrial gene expression, an additional n = 87 Illumina microarray endometrial samples from GSE141549 were analysed and combined with our RNA-seq differential expression results as part of a meta-analysis.
Techniques: Microarray, RNA Sequencing Assay